https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52189 Wed 04 Oct 2023 11:11:02 AEDT ]]> Hemin-decreased cadmium uptake in pak choi (Brassica chinensis L.) seedlings is heme oxygenase-1 dependent and relies on its by-products ferrous iron and carbon monoxide https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49467 2+ flux from pak choi roots and demonstrated that hemin treatment decreased Cd uptake rather than its translocation within plants. Moreover, through comparing the responses of different chemical treatments in pak choi seedlings and Arabidopsis wild-type and heme oxygenase-1 (HO-1) mutant, we provided evidence that hemin-decreased Cd uptake was HO-1 dependent. Furthermore, analyses of hemin degradation products suggested that the hemin-derived suppression of Cd uptake suppression was probably relying on its degradation by-products, ferrous iron (Fe²⁺) and carbon monoxide (CO), via repressing the expression of a Fe²⁺/Cd²⁺ transporter BcIRT1 in pak choi roots.]]> Thu 18 May 2023 14:34:06 AEST ]]> BcNRAMP1 promotes the absorption of cadmium and manganese in Arabidopsis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38759 in planta. BcNRAMP1 is expressed in the whole plant body of pak choi, with a higher abundance in root tissues than in shoots. Mn deficiency and Cd exposure strongly induced BcNRAMP1 transcription levels. Through transient expression of BcNRAMP1-GFP fusion protein in tobacco leaf epidermal cells, BcNRAMP1 was revealed as a plasma membrane protein. Expressing BcNRAMP1 in yeast enhanced yeast cells to absorb Mn, Cd, and iron (Fe). Overexpression of BcNRAMP1 in Arabidopsis wild-type and nramp1 mutant increased and complemented Mn and Cd transportation and accumulation, respectively. Using noninvasive microelectrode ion flux measurements, a direct evidence that BcNRAMP1 acts on Cd influx in Arabidopsis root cells was provided. The results of this study reveal that BcNRAMP1 functions as a NRAMP protein in planta, absorbing nutrient metal Mn and the toxic metal Cd.]]> Mon 24 Jan 2022 16:32:15 AEDT ]]> Mechanisms of calcium sulfate in alleviating cadmium toxicity and accumulation in pak choi seedlings https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47484 Mon 23 Jan 2023 11:48:02 AEDT ]]>